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Scientific Poster Orbitrap Eclipse RQ - Label-Free

Ultra-sensitive LC/MS workflow for in-depth label-free analysis of single mammalian cells with nanodroplet sample processing

Khatereh Motamedchaboki1, Yongzheng Cong2, Yiran Liang2, Romain Huguet1, Yufeng Shen3, Xuefei Sun1, Greg Foster1, Daniel Lopez-Ferrer1, Andreas F. Huhmer1, Ying Zhu4 and Ryan T. Kelly2,4

Hupo 2019

1 Thermo Fisher Scientific, San Jose, CA 2 Brigham Young University, Provo, UT 3 CoAnn Technologies LLC, Richland, WA 4 Pacific Northwest National Laboratory, Richland, WA, USA

In the last decade, single cell RNA sequencing has advanced our understanding of transcript heterogeneity. Currently, there are strong efforts to enable single cell proteomic analysis using mass spectrometry (MS)-based workflows. While the analysis of single-cell-sized aliquots from…

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