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Scientific Poster Orbitrap Exploris 480 Intact Antibody Characterization

Characterizations of Intact Monoclonal Antibodies by Native MS

Angela Criscuolo 1, 2; Tabiwang N. Arrey 2; Eugen Damoc2; Thomas Moehring2; Catharina Crone2 ; Martin Zeller2; Markus Kellmann2

Hupo 2019

1 Leipzig University, Leipzig, Germany 2 Thermo Fisher Scientific, Bremen, Germany

Purpose: Structural characterizations of intact monoclonal antibodies by native MS

Methods: Size exclusion chromatography and charge variant chromatography were coupled with a Thermo Scientific™ Orbitrap Exploris™
480 mass spectrometer.

Results: The two methods provided greater…

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