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Scientific Poster Orbitrap Fusion Lumos Top-Down Proteomics

High-throughput Top-down FAIMS Data Analysis with ProSightPD Nodes in Proteome Discoverer Software

Mick Greer1, Vincent Gerbasi2, Joseph Greer3, Ryan Fellers3, David Horn4, Romain Huguet4, Susan Abbatiello5, Michael Belford4, Philip Compton2, Kenneth Durbin3, Neil Kelleher2, Richard LeDuc3, Scott Peterman4, Paul Thomas2

1 Thermo Fisher Scientific, Austin, TX, 2 Northwestern University, Evanston, IL 3 Proteinaceous Inc., Evanston, IL 4 Thermo Fisher Scientific, San Jose, CA 5 Northeastern University, Boston, MA

Purpose: Evaluate Thermo Scientific™ ProSightPD™ nodes for analysis of top-down FAIMS data.

Methods: Samples were fractionated offline prior to LC separation coupled to a FAIMS enabled Orbitrap mass spectrometer. RAW files were imported directly into Thermo Scientific™ Proteome…

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