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Application Note Q Exactive HF-X, Q Exactive HF BIOPHARMACEUTICALS - ALL

Antibody subunit analysis workflow on a quadrupole-Orbitrap mass spectrometer: from optimized sample preparation to data analysis

1. Kai Scheffler 2. Eugen Damoc

APPLICATION NOTE 72854

1. Thermo Fisher Scientific, Germering 2. Thermo Fisher Scientific, Bremen


The goal of this application note is to discuss the importance of proper sample preparation for mAb subunit analysis and to provide optimized LC/MS conditions to achieve conclusive information on the mass of the subunits as well as for performing middle-down analysis for sequence…

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