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Scientific Poster Q Exactive HF-X, Q Exactive HF Targeted Quantitation

High Throughput Signaling Pathway Analysis Using Multiplex-Immunoprecipitation and Fast LC-PRM

Sebastien Gallien1,2, Aaron Gajadhar3, Bhavin Patel4, Tabiwang Arrey5, Dave Sarracino2, Sarah Trusiak2, Yue Xuan2,5, Emily Chen2

1 Thermo Fisher Scientific, Paris, France 2 Thermo Fisher Scientific, PMSC, Cambridge, MA 3 Thermo Fisher Scientific, San Jose, CA 4 Thermo Fisher Scientific, Rockford, IL 5 Thermo Fisher Scientific, Bremen, Germany High

Purpose: High-throughput mass spectrometry-based protein assays were developed, based on multiplex-immunoprecipitation, fast LC separations, and advanced PRM acquisition schemes, to support signaling pathway analysis. They were applied to the monitoring of the main protein components of…

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