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Scientific Poster Q Exactive HF Targeted Quantitation

Applications of SureQuant Pathway Panels for Quantitative Analysis of Cancer Signaling Proteins

Penny Jensen1; Bhavin Patel1; Leigh Foster1; Renuka Sabnis1; Aaron Gajadhar2; Jonathan R. Krieger3; Jiefei Tong3; Michael F. Moran3,4; Ming S. Tsao5, Rosa Viner2; Andreas Huhmer2; Kay Opperman1; John Rogers1;

1 Thermo Fisher Scientific, Rockford, IL 2 Thermo Fisher Scientific, San Jose, CA 3 The Hospital for Sick Children, Toronto, Canada 4 Department of Molecular Genetics, University of Toronto, Canada 6 Princess Margaret Cancer Centre, Toronto, Canada

Purpose: To develop a robust method for quantitation of AKT pathway proteins and benchmark against western blotting.

Methods: The SureQuantTM AKT pathway (total or phospho) panels include a multiplex immunoprecipitation (IP) and mass spectrometry (MS) sample prep module (antibodies,…

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