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Scientific Poster Orbitrap Fusion Lumos, Q Exactive HF PPCC - Cross Linking

Optimization of crosslinked peptide analysis on an Orbitrap Fusion Lumos mass spectrometer

Ryan Bomgarden1, Erum Raja1, Chris Etienne1; Fan Liu4, Albert Heck4, Mathias Mueller2, Rosa Viner3

1Thermo Fisher Scientific, Rockford, IL 2Thermo Fisher Scientific, Bremen, Germany 3Thermo Fisher Scientific, San Jose, CA 4Utrecht University, the Netherlands


Purpose: To improve identification of intra- and inter-protein interactions though analysis of chemically crosslinked peptides.

Methods: Different amine-reactive, homobifuctional crosslinkers including disuccinimidyl suberate (DSS), bis-sulfosuccinimidyl suberate (BS3), disuccinimidyl…

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