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Scientific Poster Orbitrap Fusion Lumos Bottom-up Proteomics, PTM - Phosphorylation, RQ - TMT

Evaluation of search engines for phosphopeptide identification and quantitation

Xiaoyue Jiang1, David Horn1, Ryan Bomgarden2 ,Tara Schroeder3, Rosa Viner1, Andreas FR Huhmer1

1Thermo Fisher Scientific, San Jose, CA 2Thermo Fisher Scientific, Rockford, IL 3Thermo Fisher Scientific, Somerset, NJ

Purpose: To compare different fragmentation methods including HCD, CID, EThcD for phosphopeptide identification. The performance of search engine platforms for
phosphopeptide identification and quantitation were evaluated and optimized.

Methods: Enriched and multiplexed quantitative…

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