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Scientific Poster Q Exactive Bottom-up Proteomics, BIOPHARMACEUTICALS - ALL

A robust and sensitive capillary flow LC – high-resolution accurate-mass MS platform for discovery and targeted proteomics

Alexander Boychenko1, Mike Baynham2, Stephan Meding1; Martin Ruehl1; Martin Samonig1; Remco Swart1

International Hupo 2016

1 Thermo Fisher Scientific, Germering, Germany 2 Thermo Fisher Scientific, Runcorn, UK

We systematically investigated the effect of flow rate and column ID on MS sensitivity using samples with different complexity. The sensitivity of capillary flow LC-MS (capLC-MS) was around 20 times higher at 5 μL/min in comparison with analytical flow LC-MS analysis at 450 μL/min. The…

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