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Format	Product	Application	Title	Views
Scientific Poster	Q Exactive HF-X	SureQuant Quantitation	Targeted proteomic characterization of the AKT/mTOR pathway through analysis of clinically derived samples Rowan Matney1, Kratika Singhal1, Fang Liu1, Sarah Trusiak2, Emily Chen2, Ryan D. Leib1, Allis S. Chien1 International Hupo 2020 1. Stanford University Mass Spectrometry, Stanford, CA 2. Thermo Fisher Precision Medicine Science Center, Cambridge, MA To tackle questions of differential protein expression in the expanding phosphoproteome, one emerging strategy is the targeted measurement of protein candidates from critical pathways. Here, we use a targeted sample preparation strategy (SureQuant) to enrich clinical tissue samples for proteins specific to the AKT/mTOR pathway. We compare results across two tissue types: frozen brain tissue and formalin-fixed paraffin-embedded (FFPE) brain tissue slides, as well as two control lysates: A549 (lung cancer) and MCF7 (breast cancer). This robust method allows for multiplexed immunoprecipitation in tandem with selective probing for pathway-relevant phosphorylated proteins. In this case, these targets are 30 unique peptides from 10 proteins in the AKT/mTOR pathway. This work is foundational for future studies incorporating both quantitation and the integration of genomic data. To tackle questions of differential protein expression in the expanding phosphoproteome, one emerging strategy is the targeted measurement of protein candidates from critical pathways. Here, we use a targeted sample preparation strategy (SureQuant) to enrich clinical tissue samples for…	33 Number of Views

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Application

Title

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Scientific Poster

Q Exactive HF-X

SureQuant Quantitation

Targeted proteomic characterization of the AKT/mTOR pathway through analysis of clinically derived samples

Rowan Matney1, Kratika Singhal1, Fang Liu1, Sarah Trusiak2, Emily Chen2, Ryan D. Leib1, Allis S. Chien1

International Hupo 2020

1. Stanford University Mass Spectrometry, Stanford, CA 2. Thermo Fisher Precision Medicine Science Center, Cambridge, MA

To tackle questions of differential protein expression in the expanding phosphoproteome, one emerging strategy is the targeted measurement of protein candidates from critical pathways. Here, we use a targeted sample preparation strategy (SureQuant) to enrich clinical tissue samples for…

33
Number of Views

Scientific Library

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Targeted proteomic characterization of the AKT/mTOR pathway through analysis of clinically derived samples

Rowan Matney1, Kratika Singhal1, Fang Liu1, Sarah Trusiak2, Emily Chen2, Ryan D. Leib1, Allis S. Chien1

Scientific Library

Access peer-reviewed scientific papers, application notes, technical guides, specification sheets, scientific posters, brochures, product support bulletins, webinars, and much more....

Optional: refine the library search results by searching the entire document for a keyword or exact phrase of interest. Use the additional tabs to further refine the library search results.

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Optional: refine the library search results by indicating the applications of interest. Use the additional tabs to further refine the library search results.

Optional: refine the library search results by indicating the specific Orbitrap-based platform of interest. Use the additional tabs to further refine the library search results.

Optional: refine the library search results using these filters. Use the additional tabs to further refine the library search results.

Targeted proteomic characterization of the AKT/mTOR pathway through analysis of clinically derived samples

Rowan Matney1, Kratika Singhal1, Fang Liu1, Sarah Trusiak2, Emily Chen2, Ryan D. Leib1, Allis S. Chien1

Optional: refine the library search results by searching the entire document for a keyword or exact phrase of interest.
Use the additional tabs to further refine the library search results.

Optional: refine the library search results by indicating the format of interest.
Use the additional tabs to further refine the library search results.

Optional: refine the library search results by indicating the applications of interest.
Use the additional tabs to further refine the library search results.

Optional: refine the library search results by indicating the specific Orbitrap-based platform of interest.
Use the additional tabs to further refine the library search results.

Optional: refine the library search results using these filters.
Use the additional tabs to further refine the library search results.