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Scientific Poster Orbitrap Exploris 480 SureQuant Quantitation, PTM - Phosphorylation

Multi-pathway signaling analysis using a synthetic phosphopeptide panel, standardized sample preparation kits and SureQuant internal standard targeted quantitation

Aaron S. Gajadhar1, Bhavin Patel2, Penny Jensen2, Sebastien Gallien3,4, Romain Huguet1, Kay Opperman2, John C Rogers2, Andreas Huhmer1, Daniel Lopez-Ferrer1

International Hupo 2020

1Thermo Fisher Scientific, San Jose, CA; 2Thermo Fisher Scientific, Rockford, IL; 3Thermo Fisher Scientific, PMSC, Cambridge, MA; 4Thermo Fisher Scientific, Paris, France.

To develop a universal phosphopeptide enrichment and internal standard (IS)-guided LC-MS acquisition workflow for reproducible, sensitive and high-density absolute quantification of biologically relevant phosphorylation sites in multiple biological pathways.

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