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Scientific Poster Any Orbitrap Bottom-up Proteomics

Boosting the number of peptide identifications and their confidence using INFERYS Rescoring

Dave Horn3, Daniel Lopez Ferrer3, Bernard Delanghe2, Daniel Zolg1, Martin Frejno1

International Hupo 2020

1MSAID GmbH, Garching, Germany; 2Thermo Fisher Scientific (Bremen) GmbH, Bremen, Germany; 3Thermo Fisher, San Jose, CA

Most database search algorithms compare experimental fragmentation spectra of peptides with lists of theoretical fragment masses corresponding to peptides from an in silico digest of a protein database to calculate similarity measures, while largely disregarding the intensity dimension.…

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