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Scientific Poster Orbitrap Eclipse RQ - TMT

Extending Capabilities of Real-Time Database Search on the Orbitrap Eclipse Tribrid Mass Spectrometer for Multiplexed Proteomics

Yang Liu, William D. Barshop, Amirmansoor Hakimi, Jesse D. Canterbury, Romain Huguet, Rosa Viner

International Hupo 2020

Thermo Fisher Scientific, San Jose, CA, USA

To evaluate recent improvements to Real-Time Search for TMT SPS MS3 quantitation workflow including: 1) Close-out to prevent acquisition of excess MS3 for proteins which have already reached a sufficient depth of quantitative sampling, 2) Online false discovery rate (FDR) assessments to…

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