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Orbitrap Eclipse, Q Exactive HF
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SureQuant Quantitation
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SureQuant targeted MS workflow for Quantitative analysis of Ras and AKT signaling pathways
Penny Jensen1, Bhavin Patel1, Aaron S. Gajadhar2, Sebastien Gallien3,4, Andreas Huhmer2, Daniel Lopez-Ferrer2, Kay Opperman1, John C. Rogers
International Hupo 2020 1Thermo Fisher Scientific, Rockford, IL
2Thermo Fisher Scientific, San Jose, CA
3Thermo Fisher Scientific, PMSC, Cambridge, MA
4Thermo Fisher Scientific, Paris, France.
The RAS/MAPK and AKT/mTOR pathways represent key mechanisms for cells to regulate cell survival, proliferation, and motility. The cross-talk between two pathways plays a central role in tumor progression and anti-cancer drug resistance. The quantitation of pathway protein expression and modifications are critical for characterization of disease, monitoring cancer progression and determining treatment response. A major limitation in the quantitation of pathway proteins is the lack of rigorously validated methods/reagents and a reliance on semi-quantitative results from Western blotting. We have utilized a novel SureQuant internal standard (IS)-triggered method applying a pool of reference internal standards to quantitate abundance of pathway proteins in a single MS run.
The RAS/MAPK and AKT/mTOR pathways represent key mechanisms for cells to regulate cell survival, proliferation, and motility. The cross-talk between two pathways plays a central role in tumor progression and anti-cancer drug resistance. The quantitation of pathway protein expression and…
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