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Scientific Poster Orbitrap Eclipse PTM - Glycosylation

Comprehensive Analysis of IgE Glycoforms by FAIMS-LC-MS/MS using Orbitrap Eclipse Mass Spectrometer

Aman Makaju1; Kim Alving2; Bing Wang2; Rosa Viner1

ASMS 2020

1ThermoFisher Scientific, San Jose, CA; 2Sanofi, Waltham, MA

Purpose: Correctly and confidently identify N-glycosylation sites and its glycoforms in a biologic using High-Field Asymmetric Waveform Ion Mobility Spectrometry System (FAIMS Pro) interfaced to the Orbitrap Eclipse Mass Spectrometer.

Methods: LC/MS analysis was performed on Thermo…

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