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Scientific Poster Q Exactive HF RQ - TMT

An Optimized Sample Preparation Method of Formalin-Fixed Paraffin-Embedded Tissues for Mass Spec Applications

Kara Zehr1; Bhavin Patel2; Amarjeet Flora2; Penny Jensen2; Sergei Snovida2; Ryan Bomgarden2; Kay Opperman2; John C Rogers2

ASMS 2020

1 University of Illinois at Urbana-Champaign, Champaign, IL 2 Thermo Fisher Scientific, Rockford, IL


Purpose: To optimize a protocol for extracting proteins from FFPE tissues compatible with MS sample prep and LC-MS/MS analysis.

Method: We evaluated different paraffin removal protocols and lysis/homogenization techniques using FFPE sections from normal and tumor breast, lung, and…

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