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Scientific Poster Orbitrap Exploris 480 PTM - Phosphorylation

Turnkey, Multi-pathway Signaling Analysis Using a Synthetic Phosphopeptide Panel, Standardized Sample Preparation Kits and SureQuant Internal Standard Targeted Quantitation

Aaron S. Gajadhar1; Bhavin Patel2; Penny Jensen2; Sebastien Gallien3,4; Romain Huguet1; Kay Opperman2; John C Rogers2; Andreas Huhmer1; Daniel Lopez-Ferrer1

ASMS 2020

1Thermo Fisher Scientific, San Jose, CA; 2Thermo Fisher Scientific, Rockford, IL; 3Thermo Fisher Scientific, PMSC, Cambridge, MA; 4Thermo Fisher Scientific, Paris, France


Purpose: We sought to develop a universal phosphopeptide enrichment and internal standard (IS)-guided LC-MS acquisition workflow for reproducible, sensitive and high-density absolute quantification of biologically relevant phosphorylation sites in multiple biological pathways.

Methods:…

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