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Application Note Orbitrap Eclipse Relative Quantitation, RQ - TMT

High-throughput single cell proteomics analysis with nanodroplet sample processing, multiplex TMT labeling, and ultra-sensitive LC-MS

Khatereh Motamedchaboki1 , Maowei Dou1 , Yongzheng Cong2 , Romain Huguet1 , Aaron M Robitaille1 , Yufeng Shen3 , Geremy Clair4 ,Charles Ansong4 , Daniel Lopez-Ferrer1 , Ryan T. Kelly2,4, and Ying Zhu4


1 Thermo Fisher Scientific, San Jose, CA, 2 Brigham Young University, Provo, UT, 3 CoAnn Technologies LLC, Richland, WA, 4 Pacific Northwest National Laboratory, Richland, WA, USA

Understanding heterogeneity at the single cell level is of great interest for biomedical research. Mass spectrometry (MS) based proteomics is a promising technique for single cell analysis by enabling the identification and quantification of thousands of proteins in an unbiased manner.…

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