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Poster Note Orbitrap Fusion Lumos Top-Down Proteomics

Optimizing Top Down Analysis of Proteins on Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer

Sharma S, Mallick P, Stoyanova T, Mullen C, Weisbrod C, Canterbury J, Horn D, Zabrouskov V.

ASMS 2015 Poster Note 64439

Thermo Fisher Scientific, Stanford University, and University of California

Purpose: In this work we optimize top down analysis of intact proteins with the goal of maximizing sequence coverage on a Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer
Methods: Intact proteins were characterized using ETD High Dynamic range (ETD HD), HCD,…

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