Orbitrap Illustration

Instrument Control Software

You can use the instrument control software to collect high-quality mass spectrometry data on the Thermo Scientific mass spectrometers. Control of the instruments is through two application packages: Tune and Method Editor.

For questions about the software, to request features, or to report defects, please email planet.orbitrap@thermofisher.com.

Orbitrap Tribrid MS Series Instrument Control Software Version 3.4

 

Release date: September 22, 2020
Build number: 3.4.3072.18
Instruments: Orbitrap Eclipse MS, Orbitrap Fusion Lumos MS, Orbitrap Fusion MS, and Orbitrap ID-X MS

Software requirements: Xcalibur 4.3 or later (use 4.4 for the latest AcquireX features).  Note, use of Orbitrap Tribrid Series 3.4 requires Windows 10.  Please see the Update Overview for Windows 10 PC information. 


 

Follow the upgrade instructions provided: Orbitrap Tribrid MS Series ICSW v. 3.4 Release Notes
 

Download the software: Orbitrap Tribrid MS Series ICSW v. 3.4 and Xcalibur 4.4 Software

Download the Update OverviewOrbitrap Tribrid MS Series ICSW v.3.4 Overview


New Features



The Orbitrap Tribrid MS Series instrument control software version 3.4 incorporates the following new and improved features:
  • (Orbitrap Eclipse only) Support for Real-Time Search with multiple FAIMS CVs, neutral loss option, “closeout” option, and FDR-based filtering
  • (Orbitrap Eclipse only) Support for PTCR with tMS3 experiments
  • RunStart EASY-IC mass calibration performs internal mass correction for each selected run without having to perform EASY-IC per individual spectrum
  • Time dependent gas mode provides flexibility for gas settings during the method
  • Isotope exclusion filter permits isotope exclusion without having to use the dynamic exclusion filter
  • “Block Trigger” option lets the user prevent dependent scans from triggering when specific ions or ion patterns are detected (that is, opposite of standard trigger function)
  • Full MS microscans are acquired in parallel during method runs
  • Stepped collision energy parameter allows 2 to 5 collision energy levels for HCD instead of requiring 3
  • Dynamic Exclusion filter supports use of a shared exclusion list that can reduce redundant triggering between experiments (that is, in the case of a FAIMS multi-CV method)
  • MIPS filter can be placed selectively per outcome, when multiple dependent scans are present
  • (Intact Protein mode only) Automatic adjustment of the dynamic exclusion tolerances based upon whether the precursor ion was annotated with the low resolution and high resolution APD algorithm
  • Usability enhancements:
    • Support for auto-calibration including a new “Check, calibrate if required” mode, which automates the expert user behavior for quickly checking calibrations and calibrating only those items needed, which are based on the time passed since the previous calibration or Pass/Fail status.
    • Support for iAPI agreement and license access directly through the Tune application interface
    • Support for automated service team notification from Almanac, when acute system issues occur
    • Support for corrective action notifications from Almanac
    • Support for web-based method template updates
    • Improved default settings for small molecule application mode


Known Issues



  • When using Windows 10, the View Raw File button does not open the raw file properly, nor can you double-click the raw file to open it.
    • Instead, navigate to the raw file, right-click it, and browse to the appropriate application (FreeStyle) to open the file. Select the checkbox to always open a raw file with the selected application, which then becomes the default. (Reported in Release Notes 3.0 and 3.1)  
  • A patch is available here to fix a communication error in software version 3.4 associated with the UVPD laser controller, leading to a CLOL fault.
    • Instructions to fix this issue are available here.
  • A patch is available here to fix an issue in software version 3.4 associated with the coarse calibration adjustment.
    • Instructions to fix this issue are available here.
  • A patch is available here to works around a problem in software version 3.4 in which split peaks could be observed only after instrument bootup.
    • Instructions to fix this issue are available here.
  • A patch is available here to address an issue associated with possible signal intensity fluctuation under specific acquisition conditions.
    • Instructions to fix the issue are available here.
  • A patch is available here to address shortcomings in IC Make-up Flow calibration.
    • Instructions to fix the issue are available here.
  • A patch is available here to address an issue associated with instrument methods including data dependent MS2 triggered MS2 acquisition using UVPD activation.
    • Instructions to fix the issue are available here.
  • When creating a new acquisition method from a previously saved method, if the “Internal Mass Calibration” setting is switched from “user Defined Lock Mass” in the previously saved method to “Off” in the newly created acquisition method, the application of the “user defined lock mass” remains active in subsequent runs performed with the new acquisition method. Please write here in order to get a new build (including fixed defect) of Orbitrap Tribrid MS Series instrument control software version 3.4.

  • Register for Orbitrap Tribrid Series MS Technical updates and to receive alerts about newly discovered defects. 

Fixed Defects




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