Orbitrap Illustration

Horse Urine Screening

Screening and quantitation of a wide range of drugs in horse urine is challenging due to the presence of many similar molecules in the sample, the matrix interference, and the significant sample-to-sample differences due to the varying nature of the matrix. For faster analysis time, very high resolution, accurate-mass measurements and fast polarity switching are necessary to identify the drugs and metabolites.
The workflow described here supports solid-phase extraction (SPE), liquid-liquid extraction (LLE), and online sample extraction using Thermo Scientific TurboFlow technology for many classes of drugs and metabolites. It uses simple full-scan MS at 100,000 FWHM resolution in alternating positive/negative polarity and HCD fragmentation for screening and targeted MS2 for quantitation. The MS and fragmentation data acquired in Full Scan mode is processed by Thermo Scientific ExactFinder software for screening and Thermo Scientific TraceFinder software for quantitation. ExactFinder™ software supports both targeted and unknown screening. It does a very thorough interrogation of the data by making use of the built-in database and mass spectral library of over 1400 compounds, retention times, isotope pattern matching, elemental composition determinations and ChemSpider searches to identify and confirm drugs and metabolites in the sample.

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Workflow Overview for Horse Urine Screening

Screening of banned substances in horse urine is a challenging task. Horse urine is one of the most complex matrices for analytical methods. Further, the number of drugs and other substances that need to be monitored is constantly growing. Methodologies that enable screening for potentially everything are needed to improve the efficiency of analytical labs.
The workflow described here uses a solid-phase extraction (SPE) or liquid-liquid extraction (LLE) method for sample preparation. It uses full-scan MS at 70,000 to 100,000 FWHM resolution.

Literature Highlights


Use of benchtop exactive high resolution and high mass accuracy orbitrap mass spectrometer for screening in horse doping control

Moulard Y, Bailly-Chouriberry L, et al.
Anal Chim Acta. 2011 Aug 26;700(1-2):126-36.

High resolution accurate mass screening of prohibited substances in equine plasma using liquid chromatography – Orbitrap mass spectrometry.

Ho EN, Kwok WH, et al.
Drug Test Anal. 2012 Sep 3.