Orbitrap Illustration


Bioanalysis, the study of the concentration of components of interest in biological matrices, covers a range of applications from small molecule discovery PK quantitation to regulated bioanalysis in toxicological and human clinical studies of large and small molecule therapeutics.

Workflow Overview for Bioanalysis

Pharmacokinetics (PK) studies the distribution of an externally administered compound or drug candidate inside a living animal. The primary endpoint is the calculation of various important pharmacokinetic parameters such as bioavailability (F), exposure (as area under the curve, AUC), and clearance (CL). Although in vitro models are used to predict potential pharmacokinetic performance prior to performing the in vivo study, the PK assay is a key step in determining the quality of a potential drug candidate as well as validating in vitro/in vivo correlation (IVIVC) efforts. PK samples often consist of plasma and, therefore, require a sensitive and robust method of analysis. Data acquisition using high-resolution accurate mass analysis by full-scan MS, SIM (selected ion monitoring) or PRM (parallel reaction monitoring, see refs (1) & (2)) offers a simple alternative to traditional SRM analysis on triple quadrupoles without compromising robustness or sensitivity. In addition, added selectivity can be achieved through high resolution accurate mass selection of the desired mass. The Thermo Scientific Q Exactive™ MS provides unparalleled specificity, sensitivity, and robustness without the need for SRM optimization generally required for triple quadrupole analysis.  Additionally Q Exactive MS experiments can be designed to collect supplementary information regarding qualitative information for both known and unknown sample components without compromising primary quantitative requirements.