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Scientific Poster Orbitrap Fusion Lumos PPCC - Hydrogen-Deuterium Exchange

Protein Conformation Study by Hydrogen/Deuterium Exchange Mass Spectrometry with Electron Transfer Dissociation

Terry Zhang, Stephane Houel, Jonathan Josephs

Thermo Fisher Scientific, San Jose, CA, USA

Purpose: Study protein conformation by hydrogen/deuterium exchange mass spectrometry (HDX) using electron transfer dissociation (ETD) to get single amino acid resolution.

Methods: A model peptide was used to confirm the proper ETD experimental condition for low deuterium scrambling…

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