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Scientific Poster Orbitrap Fusion Lumos RQ - TMT, PPCC - Cross Linking

Relative Quantification of TMT-labeled, cross-linked proteins using XlinkX node in Proteome Discoverer

Rosa Viner1, Kai Fritzemeier2 , Frank Berg2 , Torsten Ueckert2 , Leigh Foster3 , Ryan Bomgarden3 , Richard A. Scheltema 4 , Albert J.R. Heck 4 , Clinton Yu5 , Lan Huang5 ;

1 Thermo Fisher Scientific, San Jose, CA 2 Thermo Fisher Scientific, Bremen, Germany 3 Thermo Fisher Scientific, Rockford, IL 4 Utrecht University, Utrecht, The Netherlands 5 University of California, Irvine, Irvine, CA


Purpose: Development of complete analytical workflow in Thermo Scientific™ Proteome Discoverer™ 2.3 software for identification and multiplex quantitation of cross-linked peptides and proteins.

Methods: Cytochrome C and rabbit 20S proteasome complex in PBS buffer (pH 7.4) or HEPES…

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