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Scientific Poster Q Exactive HF RQ - Label-Free

A streamlined workflow for high-throughput, precise, and comprehensive large-scale quantitative proteomics analysis

Yue Xuan1, 2, Yue Zhou3, Sebastien Gallien2, 4, Pedro Navarro1 , Oleksandr Boychenko5, Joshua Nicklay6, Jenny Ho7, Scott Peterman8, Ken Miller8

ASMS 2018

1) Thermo Fisher Scientific, Bremen, Germany; 2) Thermo Fisher Scientific, Precision Medicine Research Center, Cambridge, MA, USA; 3) Thermo Fisher Scientific, Shanghai, China; 4) Thermo Fisher Scientific, Paris, France; 5) Thermo Fisher Scientific, Germering, Germany; 6) Thermo Fisher Scientific, New Jersey, USA; 7) Thermo Fisher Scientific, Hemel Hempstead, UK; 8) Thermo Fisher Scientific, San Jose, USA

Purpose: A novel and streamlined high resolution MS1 based quantitation data-independent acquisition (HRMS1–DIA) workflow is developed aiming at proteomics large-cohort studies.

Methods: Experiments were performed on a capillary chromatographic separation with 1h gradient or nanoLC…

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