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Scientific Poster Q Exactive HF, Q Exactive HF-X Targeted Quantitation

High-Throughput Proteomics Quantification Enabled by Fast LC Separation and Advanced PRM Acquisition

Sebastien Gallien1,2, Aaron Gajadhar3, Bhavin Patel4, Tabiwang Arrey5, Dave Sarracino2, Yue Xuan2,5, Emily Chen2

1 Thermo Fisher Scientific, Paris, France 2 Thermo Fisher Scientific, Precision Medicine Science Center, Cambridge, MA 3 Thermo Fisher Scientific, San Jose, CA 4 Thermo Fisher Scientific, Rockford, IL 5 Thermo Fisher Scientific, Bremen, Germany

Purpose: High-throughput LC-PRM set-ups were developed, based on fast LC separations and advanced PRM acquisition schemes, to support proteomics studies. They were applied to the monitoring of the main protein components of AKT/mTOR signaling pathway enriched or not through multiplex…

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