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Scientific Poster Q Exactive HF-X Bottom-up Proteomics, RQ - Label-Free

Optimized DDA+ and HR-DIA workflows for standardized, reproducible, precise and robust label-free quantitation of proteomes

Aaron S. Gajadhar1, Oleksandr Boychenko2, Xin Zhang3, Yue Xuan4, and Andreas Huhmer

ASMS 2018

Thermo Fisher Scientific, San Jose1, CA, USA; Germering, Germany2; Sunnyvale, CA3, USA; Bremen, Germany4

Purpose: To overcome the limitations of current label-free proteome quantitation (LFQ) protocols, we sought to develop an end-to-end, complete LFQ workflow solution for improved robustness, reproducibility, and throughput while still retaining high-performance.

Methods: A…

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