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Scientific Poster Orbitrap Fusion Lumos Bottom-up Proteomics

Development of a Robust and Reproducible Global Plasma Proteome Profiling Workflow for Clinical Research

Scott Peterman1, Emily Chen1, David Sarracino1, Amol Prakash2, and Ken Miller1

ASMS 2018

1 Thermo Fisher Scientific PMSC, Cambridge, MA, USA 2 Optys Technologies, Shrewsbury, MA, USA

Purpose: Increase the routine plasma proteome profiling capabilities while maintaining 1 hour sample injection cycles and maximizing system robustness.

Methods: The developed method modified the chromatographic separation by incorporating analytical flow UHPLC pumps, a PLRP-S trapping…

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