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Scientific Poster Orbitrap Fusion Bottom-up Proteomics, Targeted Quantitation

Diagnosis of Ecosystem Adaptive Responses by Analysis of Hundreds of Peptide Biomarkers in the North Atlantic Ocean using Targeted Metaproteomics

Mak Saito1, Matt McIlvin1, Dawn Moran1, Luis Valentin1, Romain Huguet2, Graeme McAlister2, Shannon Eliuk2, Rod Johnson3

ASMS 2017 Poster

1 Marine Chemistry and Geochemistry Department, Woods Hole Oceanographic Institution 2 Thermo Fisher Scientific San Jose 3 Bermuda Institute of Ocean Sciences


Motivation
● Ocean microbes are Earth’s life support system
● Developing Targeted metaproteomics to detect and diagnose long-term ecosystem and biogeochemical changes
● Absolute concentrations are necessary because biological matrix is not constant
● Proof-of-concept at…

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