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Scientific Paper Orbitrap Fusion Lumos Bottom-up Proteomics, PTM - Phosphorylation

Improved Precursor Characterization for Data-Dependent Mass Spectrometry

Alexander S. Hebert1, Christian Thöing4, Nicholas M. Riley2, Nicholas W Kwiecien1, Evgenia Shiskova3, Romain Huguet5, Helene L. Cardasis5, Andreas Kuehn4, Shannon Eliuk5, Vlad Zabrouskov5, Michael S. Westphall1, Graeme C. McAlister5, Joshua J. Coon1,2,3,6*

Anal. Chem., Just Accepted Manuscript

1Genome Center of Wisconsin, Departments of 2Chemistry and 3Biomolecular Chemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA 4Thermo Fisher Scientific, 28199, Bremen, Germany 5Thermo Fisher Scientific, San Jose, CA 95134, US 6Morgridge Institute for Research, Madison, WI, 53406 USA


Modern ion trap mass spectrometers are capable of collecting up to 60 tandem MS (MS/MS) scans per second, in theory providing acquisition speeds that can sample every eluting peptide precursor presented to the MS system. In practice, however, the precursor sampling capacity enabled by…

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