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Scientific Paper Q Exactive HF-X, Q Exactive HF Bottom-up Proteomics, PTM - Phosphorylation, RQ - TMT, RQ - Label-Free

Performance evaluation of the Q Exactive HF-X for shotgun proteomics

Christian D. Kelstrup1, Dorte B. Bekker-Jensen1, Tabiwang N. Arrey2, Alexander Hogrebe1, Alexander Harder2, Jesper V. Olsen*,1

J. Proteome Res., Just Accepted Manuscript

1) The Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, DENMARK 2) Thermo Fisher Scientific, Bremen, GERMANY


Progress in proteomics is mainly driven by advances in mass spectrometric (MS) technologies. Here we benchmarked the performance of the latest MS instrument in the benchtop Orbitrap series, the Q Exactive HF-X, against its predecessor for proteomics applications. A new peak picking…

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