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Scientific Paper Q Exactive HF Untargeted Metabolomics

Advantage of High Resolution Accurate Mass Spectrometry for Metabolite Identification in Untargeted Metabolomics Studies

Ioanna Ntai, Ralf Tautenhahn, Tim Stratton, Anastasia Kalli, Amanda Souza, Andreas Hühmer

Thermo Fisher Scientific, San Jose, CA, United States

Purpose: Determine the mass spectral resolution required to detect and identify the highest number of compounds from a complex sample, such as human plasma, during a 15min LC/MS run.

Methods: Human plasma (NIST SRM 1950) was analyzed at different mass spectral resolutions ranging from…

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