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Scientific Poster Q Exactive HF, Q Exactive HF-X Bottom-up Proteomics, Relative Quantitation

Revolutionary Proteome Profiling and Quantitation without Compromising Speed, Sensitivity, and Selectivity

Yue Xuan1, Oleksandr Boychenko2, Remco Swart2, Alexander Harder1, Thomas Moehring1

ASMS 2017 Poster

1) Thermo Fisher Scientific, Bremen, Germany 2) Thermo Fisher Scientific, Germering, Germany

Purpose: A novel high resolution MS1 based DIA method was developed to comprehensively, reproducibly, and precisely quantify proteins in complex matrices.

Methods: Instead of quantifying the peptides on MS2 intensities, MS1 scans offer potentially higher sensitivity since the peptide…

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