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Scientific Poster Q Exactive Glycan Identification & Characterization

Unraveling the Complexity of Sulfated O-glycans from Mucins by Anion Exchange Chromatography-Orbitrap Mass Spectrometry

Zoltan Szabo1, Niclas G. Karlsson2, Yury Agroskin1, Jim Thayer1, Gina Tan3, Rosa Viner3, Andreas Huhmer3, Jeff Rohrer1, Liu Yan1, Kannan Srinivasan1, Dietmar Reusch4 and Christopher A. Pohl1

ASMS 2017 Poster

1 ThermoFisher Scientific, Sunnyvale, CA, USA 2 University of Gothenburg, Gothenburg, Sweden 3 ThermoFisher Scientific, San Jose, CA, USA, 4 Roche Diagnostics GmbH, Penzberg, Germany

In this study, High Performance Anion Exchange Chromatography (HPAE) coupled to an HRAM Orbitrap™ mass spectrometer is demonstrated to be an efficient tool to separate and sequence sulfated O-glycan alditols. Porcine gastric mucin (type III) harbors high levels of sulfated but fewer…

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