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Scientific Paper Orbitrap Fusion Lumos PTM - Phosphorylation

Phosphoproteomics with Activated Ion Electron Transfer Dissociation

Nicholas M. Riley,1,2 Alexander S. Hebert,1 Gerhard D├╝rnberger,3,4,5 Florian Stanek,3 Karl Mechtler,3,5 Michael S. Westphall,1 and Joshua J. Coon1,2,6,7

Anal. Chem., Just Accepted Manuscript

1Genome Center of Wisconsin, Departments of 2Chemistry and 6Biomolecular Chemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA 3Institute of Molecular Pathology (IMP), Campus-Vienna-Biocenter 1, A-1030 Vienna, Austria 4GMI, Gregor Mendel Institute of Molecular Plant Biology, Dr. Bohr Gasse 3, A-1030 Vienna, Austria 5IMBA, Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Dr. Bohr Gasse 3, A-1030 Vienna, Austria 7Morgridge Institute for Research, Madison, Wisconsin, USA


The ability to localize phosphosites to specific amino acid residues is crucial to translating phosphoproteomic data into biological meaningful contexts. In a companion manuscript we described a new implementation of activated ion electron transfer dissociation (AI-ETD) on a…

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