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Scientific Paper Orbitrap Fusion Lumos Bottom-up Proteomics

Implementation of Activated Ion Electron Transfer Dissociation on a quadrupole-Orbitrap-linear ion trap hybrid mass spectrometer

Nicholas M. Riley,1,2 Michael S. Westphall,1 Alexander S. Hebert,1 and Joshua J. Coon1-4,*

Anal. Chem., Just Accepted Manuscript

1 Genome Center of Wisconsin, Departments of 2Chemistry and 3Biomolecular Chemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA 4Morgridge Institute for Research, Madison, Wisconsin, USA

Using concurrent IR photo-activation during electron transfer dissociation (ETD) reactions, i.e., activated ion ETD (AI-ETD), significantly increases dissociation efficiency resulting in improved overall performance. Here we describe the first implementation of AI-ETD on a…

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