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Scientific Paper Orbitrap Elite Bottom-up Proteomics

An Approach to Spatiotemporally Resolve Protein Interaction Networks in Living Cells

Braden T. Lobingier,1,8 Ruth Hu¨ ttenhain,2,3,4,8 Kelsie Eichel,5 Kenneth B. Miller,6 Alice Y. Ting,7 Mark von Zastrow,1,2,9,* and Nevan J. Krogan2,3,4,

Cell Volume 169, Issue 2, p350–360.e12, 6 April 2017

1 Department of Psychiatry, University of California, San Francisco, San Francisco, CA 94158, USA 2 Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA 3 Quantitative Biosciences Institute, QBI, University of California, San Francisco, San Francisco, CA 94158, USA 4 J. David Gladstone Institutes, San Francisco, CA 94158, USA 5 Program in Biochemistry and Molecular Biology, University of California, San Francisco, San Francisco, CA 94158, USA 6 Thermo Fisher Scientific, San Jose, CA 95134, USA 7 Departments of Genetics, Biology, and Chemistry, Stanford University, Stanford, CA 94305, USA 8 Co-first author 9 Lead Contact


Cells operate through protein interaction networks organized in space and time. Here, we describe an approach to resolve both dimensions simultaneously by using proximity labeling mediated by engineered ascorbic acid peroxidase (APEX). APEX has been used to capture entire organelle…

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