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Scientific Paper Orbitrap Fusion Lumos, Q Exactive HF, Q Exactive Plus Top-Down Proteomics, Intact Protein Characterization

High resolution top-down experimental strategies on the Orbitrap platform

Kai Scheffler, Rosa Viner, Eugen Damoc

Journal of Proteomics Available online 3 April 2017 In Press, Accepted Manuscript

a Thermo Fisher Scientific, Im Steingrund 4-6, 63303 Dreieich, Germany b Thermo Fisher Scientific, 655 River Oaks Parkway, San Jose, CA, USA c Thermo Fisher Scientific, Hanna-Kunath-Str. 11, 21899 Bremen, Germany

Top-down mass spectrometry (MS) strategies allow in-depth characterization of proteins by fragmentation of the entire molecule(s) inside a mass spectrometer without requiring prior proteolytic digestion. Importantly, the fragmentation techniques on commercially available mass…

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