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Scientific Paper Exactive Plus EMR Intact Protein Characterization, Intact Antibody Characterization

Precise quantification of mixtures of bispecific IgG produced in single host cells by liquid chromatography-Orbitrap high-resolution mass spectrometry

Yiyuan Yin, Guanghui Han, Jianhui Zhou, Michael Dillon, Luke McCarty, Lou Gavino, Diego Ellerman, Christoph Spiess, Wendy Sandoval & Paul J. Carter

MABS 2016, VOL. 8, NO. 8, 1467–1476

a. Antibody Engineering Department, Genentech, Inc., South San Francisco, CA, USA b. Proteomics and Biologics Resources Department, Genentech, Inc., South San Francisco, CA, USA c. Protein Chemistry and Structural Biology Department, Genentech, Inc., South San Francisco, CA, USA d. Chromatography and Mass Spectrometry Division, Thermo Fisher Scientific, San Jose, CA, USA

Bispecific IgG are heterotetramers comprising 2 pairs of heavy and light chains. Co-expression of the 4 component chains in a single host cell typically yields the desired bispecific IgG plus up to 9 additional incorrect chain pairings. Several protein engineering strategies have been…

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