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Glycan Quantitation

The field of glycomics is transitioning from qualitative to quantitative, where the focus is on the biological insights into the systems being studied. These functional insights often require comparing relative glycan abundance between two or more biological states to elucidate the role of glycans in a disease or to generate lead candidates as part of biomarker discovery programs. Mass spectrometry has emerged as a key technology for the identification of glycans from a wide range of biological sources. Here we describe techniques for relative quantitation of glycans using mass spectrometry.

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Workflow Overview for 2-AA Quantitation

 

Glycan quantitation has seen a number of method developments in recent years. One such approach takes advantage of reductive amination to introduce stable isotope labels onto the glycans.  Similar to the 2-AB label used in glycan characterization, the 2-aminobenzoic acid (2-AA) label offers many of the same benefits for LC-MS analysis. However, it also available commercially as stable isotopic 12[C6]-2-aminobenzoic acid (12[C6]-2-AA) and 13[C6]-2-AA variants and thus can be used for relative quantitation experiments.[1]


 





 

 



References


1. Mass spectrometric-based stable isotopic 2-aminobenzoic acid glycan mapping for rapid glycan screening of biotherapeutics

Prien, J.M., Prater, B.D., Qin, Q. and Cockrill, S.
Anal.  Chem. 2010, 82, 1498-1508.