While analysis of denatured proteins may be the most common LC/MS protein analysis, many biopharmaceutical and biological research applications require analysis of proteins in their native (intact) state. With the optional extended mass range (EMR) of up to m/z 20,000 and improved detection of high-mass ions, the Thermo Scientific™ Exactive™ Plus EMR mass spectrometer is an outstanding platform for biopharmaceutical and biological research requiring analysis of monoclonal antibodies, antibody drug conjugates, protein assemblies, and other proteins in their native states. Combining unsurpassed high-resolution accurate-mass Thermo Scientific™ Orbitrap™ analysis with the extended mass range option, the Exactive Plus EMR MS is an outstanding tool for investigating the structure, topology, and architecture of native-like tertiary and quaternary protein structures.
The Exactive Plus EMR system retains all of the capabilities that make an Exactive Plus mass spectrometer a superb tool for compound identification and high-throughput, high-performance screening for targets such as impurities in monoclonal antibodies, antibody-drug conjugates (ADC), PEG-ylated proteins, oligomerized protein-based drugs, glycoforms, and protein assemblies.
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Schematic of the Exactive Plus EMR
Unique Features of the Exactive Plus EMR
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Thermo Scientific Exactive Plus Orbitrap LC-MS System: Powerful screening and quantitation
- Extended m/z range of 350–20,000
- Improved transmission of higher-mass ions for stronger signals
- Modified HCD cell pressure and controls for easy optimization of experimental conditions
- Access to short transients for improved signal-to-noise ratios
Performance Characteristics of the Exactive Plus EMR
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Max Resolving Power
up to 140,000 (FWHM) @ m/z 200
Max Scan Rate
Up to 12 Hz at mass resolution setting of 17,500 @ m/z 200
Internal: < 1 ppm RMS
External: < 3 ppm RMS
under defined conditions
External: <3 ppm RMS for m/z 350–6000
External: <5 ppm RMS for m/z 6001–10,000
External: <10 ppm RMS for m/z 10,001–15,000
In Full MS mode: 500 fg Buspirone on column S/N 100:1 acquired with H-ESI II probe
> 5000:1 in single scan, intra-scan dynamic range
One full cycle in < 1 sec
(one full-scan positive ion mode and negative ion mode each at mass resolution setting of 35,000 @ m/z 200)
Key Applications of the Exactive Plus EMR
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Customer Testimonials on the Exactive Plus EMR
“The Exactive Plus EMR Orbitrap mass spectrometer is a versatile instrument adapted not only for routine protein therapeutics analysis but also for in-depth characterization of different types of noncovalent assemblies. Combining high resolution and sensitivity, this new technology will push Native MS one step forward and offer new perspectives”
– Dr. Sarah Cianferani, CNRS - IPHC
“The Exactive Plus EMR allows us to characterize native protein complexes with superior sensitivity and mass resolving power. We expect to use it as an essential tool for deciphering molecular mechanisms of protein machine assembling and complex/drug interactions. It helps to provide an additional dimension of information beyond traditional proteomics methodologies“
– Prof. Shenheng Guan, UCSF School of Pharmacy
“The Exactive Plus EMR brings the high sensitivity and resolution associated with the Orbitrap analyser to the field of native mass spectrometry. This results in very high performance analysis of systems such as protein-ligand complexes, giving rise to exciting new possibilities for protein-fragment screening within drug discovery programmes."
– Dr. Hannah Maple, UCB Pharma
"The impact of this high mass resolving power at very high sensitivity is tremendous;
it opens up avenues to measure not only protein-protein interactions, but also covalent and non-covalent binding of small molecules to protein assemblies"
– Prof. Albert Heck, Utrecht University
Exactive Plus EMR Scientific Resources
Thermo Scientific Research
Berube M, Dibussolo J, Lafontaine C, Shi Y, Espourteille F.
Van Natta K, Kozak M, Nimkar S, Clarke W.
Van Natta K, Kozak M, He X.
Berube M, DiBussolo J, Lafontaine C, Shi Y
In-depth qualitative and quantitative analysis of composite glycosylation profiles and other micro-heterogeneity on intact monoclonal antibodies by high-resolution native mass spectrometry using a modified Orbitrap.
Rosati S, van den Bremer ET, Schuurman J, Parren PW, Kamerling JP, Heck AJ.
Rosati S, Rose RJ, Thompson NJ, van Duijn E, Damoc E, Denisov E, Makarov A, Heck AJ.
Murphy K, Bromirski M, Espourteille FA.
Beck J, Yang C, Ghosh D, Akervik K.
Yang C, Wang L, Ghosh D, Beck J, Humphries J.
Scheibner O, Bromirski M.
Scheibner O, Bromirski M.