Unique Features of the Exactive Plus
The Exactive Plus Orbitrap LC-MS is a benchtop full scan MS system designed for compound identification, high-throughput and high-performance screening and for qualitative and quantitative analysis. The Exactive Plus delivers high resolution accurate mass (HRAM) to provide fast, precise and reproducible results.
The availability of HRAM and full scan; all the data, all the time, allows retrospective data analysis for qualitative and quantitative applications. Moreover, it eliminates MS method development leading to improved and simplified analytical throughput in drug discovery laboratories.
Exactive Plus is easy-to-use, cost effective to operate and coupled with workflow-driven software, makes an ideal instrument for new users in routine analytical laboratories.
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Thermo Scientific Exactive Plus Orbitrap LC-MS System: Powerful screening and quantitation
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High Resolving Power(up to 140,000) provides precise mass accuracy for complex sample analysis
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Mass accuracy of better than 1ppm in full scan and AIF scan using fully automated AGC and mass calibration procedures
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Scan Speed operating at a 12Hz scanning frequency Exactive Plus is fully compatible with U-HPLC and ensures exact mass measurement for fast chromatography applications
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Fast Polarity Switching without sacrificing mass accuracy in any scan, a full cycle of 1 positive and 1 negative scan performed within 1 second at resolution setting of 35,000
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All-Ion-Fragmentation(AIF) with multiple dissociation techniques; in source collisional induced dissociation (CID) and high energy collisional dissociation (HCD), provides MS and MS/MS data
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Full MS scan, AIF scan, data dependent AIF, alternating Full MS and AIF scan
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Intuitive software interface
Mass Range
m/z 50-6,000
Max Resolving Power
up to 140,000 (FWHM) @ m/z 200
Max Scan Rate
Up to 12 Hz at mass resolution setting of 17,500 @ m/z 200
Mass Accuracy
Internal: < 1 ppm RMS
External: < 3 ppm RMS
under defined conditions
Sensitivity
In Full MS mode: 500 fg Buspirone on column S/N 100:1 acquired with H-ESI II probe
Dynamic Range
> 5000:1 in single scan, intra-scan dynamic range
Polarity Switching
One full cycle in < 1 sec
(one full-scan positive ion mode and negative ion mode each at mass resolution setting of 35,000 @ m/z 200)
Key Applications of the Exactive Plus
Click on the following links to be taken to the related applications workflow page.
Customer Testimonials on the Exactive Plus
“The advantages of a Exactive Plus is to screen and to quantify in one chromatographic run with exact masses, isotopic pattern and fragmentation information’s. The AIF experiments seem to be much more sensitive than with the Exactive”
– Dr. Thorsten Bernsmann, Chemisches Landes- und Staatliches Veterinäruntersuchungsamt Münster (CVUA), Germany
“Benefit of Exactive Plus (HR full scan) versus SRM in Triple Quad MS are a 'full' picture of the sample and Quan/Qual analyses”
– Dr. Bertrand Rochat, Le Centre hospitalier universitaire vaudois (CHUV), Switzerland
Exactive Plus Scientific Resources
Thermo Scientific Research
clinical research
Analysis of 25-Hydroxyvitamin D In Serum Using Automated Online Sample Preparation Technique with High Resolution Orbitrap Mass Spectrometer
Berube M, Dibussolo J, Lafontaine C, Shi Y, Espourteille F.
Quantitation of Immunosuppressant Drugs in Blood Utilizing Second Generation Exactive High Resolution Accurate Mass Spectrometer and TraceFinder Software
Van Natta K, Kozak M, Nimkar S, Clarke W.
Screening of 300 Drugs in Blood Utilizing Second Generation Exactive Plus High-Resolution, Accurate Mass Spectrometer and ExactFinder Software
Van Natta K, Kozak M, He X.
Analysis of 25-Hydroxyvitamin D in Serum Using an Automated Online Sample Preparation Technique with a High-Resolution Benchtop Orbitrap Mass Spectrometer
Berube M, DiBussolo J, Lafontaine C, Shi Y
Pharmaceuticals
High Throughput Screening in Drug Discovery for Cancer Research Peptides Using High Resolution LCMS
Murphy K, Bromirski M, Espourteille FA.
Environmental Analysis
High Resolution LC-MS for Screening and Quantitative Analysis of Antibiotics in Drinking Water Using an Orbitrap and Online Sample Preparation
Beck J, Yang C, Ghosh D, Akervik K.
Food Safety
Utilization of Rapid LC-MS for Screening and Quantitative Analysis of Pesticides in Food Matrix using an Exactive Plus Benchtop Orbitrap Platform
Yang C, Wang L, Ghosh D, Beck J, Humphries J.
Increasing Speed of UHPLC-MS Analysis Using Single-stage Orbitrap Mass Spectrometer
Scheibner O, Bromirski M.
Qualitative and Quantitative Analysis of Pesticides in Horse Feed Matrix Using Orbitrap MS
Scheibner O, Bromirski M.
