The Ultimate Screening Machine
The Thermo Scientific Exactive Plus Orbitrap MS is a benchtop LC-MS system designed for high-performance, high-throughput screening, compound identification, and quantitative analysis. Thanks to its Orbitrap™ mass analyzer, the Exactive Plus™ system delivers high-resolution, accurate-mass (HR/AM) full-scan MS for fast, precise and reproducible results and tremendous analytical confidence. The continuous acquisition of HR/AM full-scan data allows retrospective data analysis for qualitative and quantitative analyses. Moreover, it vastly simplifies MS method development. The Exactive Plus mass spectrometer features fast scanning that makes it fully compatible with narrow UHPLC peaks. It also includes fast polarity switching, which can further streamline method development and increase sample throughput. All-ions fragmentation (AIF) with multiple dissociation techniques—in source collision-induced dissociation (CID) and higher energy collisional dissociation (HCD)—adds an additional dimension of information.
The Exactive Plus mass spectrometer is easy-to-use, cost effective to operate and, coupled with workflow-driven software, makes an ideal instrument for new users and users performing largely routine analyses.
Schematic of the Exactive Plus
Unique Features of the Exactive Plus
The Exactive Plus Orbitrap LC-MS is a benchtop full scan MS system designed for compound identification, high-throughput and high-performance screening and for qualitative and quantitative analysis. The Exactive Plus delivers high resolution accurate mass (HRAM) to provide fast, precise and reproducible results.
The availability of HRAM and full scan; all the data, all the time, allows retrospective data analysis for qualitative and quantitative applications. Moreover, it eliminates MS method development leading to improved and simplified analytical throughput in drug discovery laboratories.
Exactive Plus is easy-to-use, cost effective to operate and coupled with workflow-driven software, makes an ideal instrument for new users in routine analytical laboratories.
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Thermo Scientific Exactive Plus Orbitrap LC-MS System: Powerful screening and quantitation
High Resolving Power(up to 140,000) provides precise mass accuracy for complex sample analysis
Mass accuracy of better than 1ppm in full scan and AIF scan using fully automated AGC and mass calibration procedures
Scan Speed operating at a 12Hz scanning frequency Exactive Plus is fully compatible with U-HPLC and ensures exact mass measurement for fast chromatography applications
Fast Polarity Switching without sacrificing mass accuracy in any scan, a full cycle of 1 positive and 1 negative scan performed within 1 second at resolution setting of 35,000
All-Ion-Fragmentation(AIF) with multiple dissociation techniques; in source collisional induced dissociation (CID) and high energy collisional dissociation (HCD), provides MS and MS/MS data
Full MS scan, AIF scan, data dependent AIF, alternating Full MS and AIF scan
Intuitive software interface
Performance Characteristics of the Exactive Plus
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Max Resolving Power
up to 140,000 (FWHM) @ m/z 200
Max Scan Rate
Up to 12 Hz at mass resolution setting of 17,500 @ m/z 200
Internal: < 1 ppm RMS
External: < 3 ppm RMS
under defined conditions
In Full MS mode: 500 fg Buspirone on column S/N 100:1 acquired with H-ESI II probe
> 5000:1 in single scan, intra-scan dynamic range
One full cycle in < 1 sec
(one full-scan positive ion mode and negative ion mode each at mass resolution setting of 35,000 @ m/z 200)
Key Applications of the Exactive Plus
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Customer Testimonials on the Exactive Plus
“The advantages of a Exactive Plus is to screen and to quantify in one chromatographic run with exact masses, isotopic pattern and fragmentation information’s. The AIF experiments seem to be much more sensitive than with the Exactive”
– Dr. Thorsten Bernsmann, Chemisches Landes- und Staatliches Veterinäruntersuchungsamt Münster (CVUA), Germany
“Benefit of Exactive Plus (HR full scan) versus SRM in Triple Quad MS are a 'full' picture of the sample and Quan/Qual analyses”
– Dr. Bertrand Rochat, Le Centre hospitalier universitaire vaudois (CHUV), Switzerland
Exactive Plus Scientific Resources
Thermo Scientific Research
Berube M, Dibussolo J, Lafontaine C, Shi Y, Espourteille F.
Van Natta K, Kozak M, Nimkar S, Clarke W.
Van Natta K, Kozak M, He X.
Berube M, DiBussolo J, Lafontaine C, Shi Y
Murphy K, Bromirski M, Espourteille FA.
Beck J, Yang C, Ghosh D, Akervik K.
Yang C, Wang L, Ghosh D, Beck J, Humphries J.
Scheibner O, Bromirski M.
Scheibner O, Bromirski M.